Human metabotropic glutamate receptor 2 couples to the MAP kinase cascade in chinese hamster ovary cells
作者:
T. Phillips,
A. Barnes,
S. Scott,
P. Emson,
S. Rees,
期刊:
NeuroReport
(OVID Available online 1998)
卷期:
Volume 9,
issue 10
页码: 2335-2339
ISSN:0959-4965
年代: 1998
出版商: OVID
关键词: G-protein;L-Glutamate;Metabotropic glutamate receptor (mGluR2);MAP kinase;Western blotting
数据来源: OVID
摘要:
WE have examined the functional coupling of the human metabotropic glutamate receptor type 2 (mGluR2) with the regulation of the mitogen activated protein kinase (MAP kinase) signal transduction cascade. We demonstrated that L-glutamate stimulation of the human mGluR2 receptor transiently expressed in chinese hamster ovary (CHO) cells leads to a rapid increase in the activity of p42/p44 MAP kinase (also known as the extracellular signal regulated kinases, ERK1 and ERK2). Activation of p42/p44 MAP kinase has been demonstrated in a peptide phosphorylation assay and through the demonstration of a shift in electrophoretic mobility of p42 MAP kinase following activation. In both assay systems L-glutamate stimulation of MAP kinase was inhibited by pertussis toxin and by the MEK (MAP/ERK activating kinase) inhibitor PD 98059. We conclude that L-glutamate stimulation of the mGluR2 receptor in CHO cells mediated regulation of p42/p44 MAP kinase following the activation of pertussis toxin-sensitive GαiG-proteins via a distinct protein kinase signalling pathway that utilizes MEK.
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