Activation of the retinoic acid receptorβgene by 5-aza-2’-deoxycytidine in human DLD-1 colon carcinoma cells
作者:
Sylvie Côté,
Richard Momparler,
期刊:
Anti-Cancer Drugs
(OVID Available online 1997)
卷期:
Volume 8,
issue 1
页码: 56-61
ISSN:0959-4973
年代: 1997
出版商: OVID
关键词: 5-Aza-2'-deoxycytidine;colon carcinoma;DNA methylation;retinoic acid;retinoic acid receptor β
数据来源: OVID
摘要:
We previously reported that 5-aza-2’-deoxycytldlne (5-Aza- CdR) In combination with a\\-transretinoic acid (ATRA) produced a synergistic antineoplastic effect on DLD-1 colon carcinoma cells. We also observed that 5-Aza-CdR, a potent inhibitor of DNA methylation, increased the expression of retinoic acid receptor (RAR)-β). Methylation of cytosine in the promoter-first exon region of genes has been reported to silence their expression. In an attempt to clarify the mechanism responsible for the activation of the RAR-βgene by 5-Aza-CdR in DLD-1 colon carcinoma cells, we investigated its methylation state by Southern blotting. Our results indicate that DNA hypermethylation of the RARβgene, a putative tumor suppressor gene, may be the mechanism of silencing its expression in these tumor cells. We also reported that a different schedule of 5-Aza-CdR and ATRA produced a synergistic antineoplastic effect on the colon carcinoma cells.
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