Senescence in French‐bean nodules: Occurrence of different proteolytic activities
作者:
D. Pladys,
J. Rigaud,
期刊:
Physiologia Plantarum
(WILEY Available online 1985)
卷期:
Volume 63,
issue 1
页码: 43-48
ISSN:0031-9317
年代: 1985
DOI:10.1111/j.1399-3054.1985.tb02815.x
出版商: Blackwell Publishing Ltd
关键词: Leghemoglobin;nitrogen fixation;nodules;Phaseolus vulgaris;protease;senescence
数据来源: WILEY
摘要:
A decline in nitrogenase activity (C2H2reduction) of nodules ofPhaseolus vulgarisL. cv. Contander was correlated with a decrease in their soluble protein including leghe‐moglobin. Concomitantly, two distinct proteolytic activities against leghemoglobin with acidic and alkaline pH optima were detected. The corresponding proteases were purified about 30‐fold by ammonium sulfate precipitation, gel filtration and hydroxy‐apatite chromatography. Both the acidic (pH optimum 3.5) and the alkaline (pH optimum 8.0) proteases were thiol enzymes. They were characteristic of senescing nodules, whereas only an acidic serine protease was present in functional no
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