[3H]-13-cis-Retinoic Acid Covalently Binds to Thioredoxin Reductase in Human Keratinocytes
作者:
K.U. Schallreuter,
T. Grebe,
M.R. Pittelkow,
J.M. Wood,
期刊:
Skin Pharmacology and Physiology
(Karger Available online 1991)
卷期:
Volume 4,
issue 1
页码: 14-20
ISSN:1660-5527
年代: 1991
DOI:10.1159/000210919
出版商: S. Karger AG
关键词: Thioredoxin reductase;Retinoic acid inhibitor;13-cis-Retinoic-acid-binding domain
数据来源: Karger
摘要:
13-cis-Retinoic acid is a stereospecific suicide inhibitor of thioredoxin reductase. [3H]-labeled 13-cis-retinoic acid has been used to covalently label thioredoxin reductase in human keratinocytes. The acid-soluble cytosol fraction of human keratinocytes contained three radioactive proteins labeled by the addition of high-specific-activity [3H]-13-cis-retinoic acid to cell cultures. One of these proteins was identified as cytosolic keratinocyte thioredoxin reductase by fast-protein liquid chromatography and SDS-gel radioautography. The inhibition of thioredoxin reductase by 13-cis-retinoic acid may explain the known cytostatic and teratogenic properties attributed to this retinoid.
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