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Comparison of fluorescent antibody, bacitracin susceptibility, latex agglutination, coa...
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Comparison of fluorescent antibody, bacitracin susceptibility, latex agglutination, coagglutination, and API 20S for identifying group A streptococci
作者:
R. J. Lesher,
A. E. Casiano-Colon,
期刊:
Canadian Journal of Microbiology
(NRC Available online 1985)
卷期:
Volume 31,
issue 4
页码: 335-338
ISSN:0008-4166
年代: 1985
DOI:10.1139/m85-064
出版商: NRC Research Press
数据来源: NRC
摘要:
A total of 200 beta-hemolytic streptococci, isolated from clinical specimens submitted to our laboratory, were identified as group A versus non-A using the fluorescent antibody technique (FA), bacitracin susceptibility (BBL, Difco, and Raven disks), SeroSTAT, Streptex, Phadebact, and the API 20S system. Of the 122 group A isolates, all methods except SeroSTAT and Phadebact yielded 92–99% agreement when compared with the Lancefield precipitin test. Phadebact yielded an 84% agreement and SeroSTAT changed from 83 to 98% after trypsinization. Numerous false positives were obtained and only FA (91%) and API 20S (96%) yielded better than 90% agreement on non-A identification when compared with the Lancefield test. The most false positives were obtained (45%) using the SeroSTAT reagents. Considering accuracy, our data suggests the FA technique to be the method of choice for identifying group A streptococci.
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