SEPARATION OF PROTEINS BY CAPILLARY ELECTROPHORESIS USING AN EPOXY BASED HYDROPHILIC COATING
作者:
JamesJ. Bao,
期刊:
Journal of Liquid Chromatography & Related Technologies
(Taylor Available online 2000)
卷期:
Volume 23,
issue 1
页码: 61-78
ISSN:1082-6076
年代: 2000
DOI:10.1081/JLC-100101436
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
Protein adsorption onto the inner walls of capillaries is a widely recognized problem in the separation of proteins by capillary electrophoresis. Chemical modification of the wall has been an effective method for suppressing this effect. The performance of an epoxy based hydrophilic coating has been evaluated under different experimental conditions. High efficiency separation of proteins (up to 200,000 theoretical plates per meter) was achieved using this coating. The recoveries of both cationic and anionic proteins were very high (over 90% for several positively charged proteins) on this capillary coating. The influence of both pH and potential on efficiency, resolution and peak capacity were examined.
点击下载:
PDF (331KB)
返 回