Microwaveable Toluidine Blue Stain for Surface Staining of Undecalcified Bone Sections
作者:
EurellJo Ann C.,
SterchiDiane L.,
期刊:
Journal of Histotechnology
(Taylor Available online 1994)
卷期:
Volume 17,
issue 4
页码: 357-359
ISSN:0147-8885
年代: 1994
DOI:10.1179/his.1994.17.4.357
出版商: Taylor&Francis
关键词: bone histology;microwave stain;plastic embedding;stain;toluidine blue
数据来源: Taylor
摘要:
AbstractToluidine blue stain has been widely used for staining undecalcified bone sections, but previous techniques required removal of embedding plastic or surface etching with both alcohol and acid solutions. In this study, solutions of toluidine blue at different pHs were used to stain unetched and acidetched sections. A group of the test staining solutions were heated in an effort to enhance section staining. Acid-etching prior to staining was found to be necessary to achieve bone matrix staining, but methanol etching could be eliminated if staining solutions were heated to 55–60°C. Sections stained with the heated solutions buffered to pH 7.0 or 8.0 had optimum morphology. (The J Histotechnol17:357, 1994)
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