Isoelectric focusing of the chick eye ciliary neuronotrophic factor
作者:
Marston Manthorpe,
Gilles Barbin,
Silvio Varon,
期刊:
Journal of Neuroscience Research
(WILEY Available online 1982)
卷期:
Volume 8,
issue 2‐3
页码: 233-239
ISSN:0360-4012
年代: 1982
DOI:10.1002/jnr.490080213
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: chick embryo;ciliary neuronotrophic factor (CNTF);isoelectric focusing
数据来源: WILEY
摘要:
AbstractA procedure is presented in which a crude extract from selected chick embryo intraocular tissues is submitted to analytical polyacrylamide slab gel isoelectric focusing. The extract contains a protein, ciliary neuronotrophic factor (CNTF) which can be eluted in active form from focused gels in a region occupied by only two protein bands. A “slot” technique is presented in which we demonstrate that the eluted CNTF activity focuses in the very restricted regionbetweenthe two visible bands and is not associated with either band. Silver stain‐densitometry is used to correlate staining intensity with protein concentration and from such an analysis it is concluded that the CNTF protein represents an extremely low proportion of total extract protein and that the minimum CNTF specific activity eluted from gel slices is 106trophic units per mg protein. This one‐step procedure will be used in the future to prepare highly purified CNTF for antibody gen
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