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Demonstration of Urinary Eosinophils inSchistosoma haematobium:a Comparative Study among Three Different Stains

 

作者: EltoumIsam A.,   SuliamanSaud M.,   IsmailBabiker M.,   AliMagdi M.,   HomeidaMamoun M. A.,  

 

期刊: Biotechnic&Histochemistry  (Taylor Available online 1993)
卷期: Volume 68, issue 3  

页码: 146-149

 

ISSN:1052-0295

 

年代: 1993

 

DOI:10.3109/10520299309104685

 

出版商: Taylor&Francis

 

关键词: Hansel's stain;naphthalene black;alkaline erythrocin B;eosinophiluria;S.haematobium

 

数据来源: Taylor

 

摘要:

Three stains, Hansel's stain, alkaline erythrocin B (AEB) and naphthalene black (NB), were used to demonstrate eosinophils in the urine of patients infected withSchistosoma haematobium. Hansel's stain was superior to the other two stains; it stained eosinophils bright red and their nuclei faint blue, and they were easily differentiated from neutrophils, lymphocytes, macrophages and epithelial cells. The method using AEB took longer than Hansel's stain and 10% of the specimens were lost during staining with this method. Like eosinophils, the neutrophils took up NB stain and their nuclei stained poorly with the counterstain.

 

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