Demonstration of Urinary Eosinophils inSchistosoma haematobium:a Comparative Study among Three Different Stains
作者:
EltoumIsam A.,
SuliamanSaud M.,
IsmailBabiker M.,
AliMagdi M.,
HomeidaMamoun M. A.,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1993)
卷期:
Volume 68,
issue 3
页码: 146-149
ISSN:1052-0295
年代: 1993
DOI:10.3109/10520299309104685
出版商: Taylor&Francis
关键词: Hansel's stain;naphthalene black;alkaline erythrocin B;eosinophiluria;S.haematobium
数据来源: Taylor
摘要:
Three stains, Hansel's stain, alkaline erythrocin B (AEB) and naphthalene black (NB), were used to demonstrate eosinophils in the urine of patients infected withSchistosoma haematobium. Hansel's stain was superior to the other two stains; it stained eosinophils bright red and their nuclei faint blue, and they were easily differentiated from neutrophils, lymphocytes, macrophages and epithelial cells. The method using AEB took longer than Hansel's stain and 10% of the specimens were lost during staining with this method. Like eosinophils, the neutrophils took up NB stain and their nuclei stained poorly with the counterstain.
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