On the Use of Laser for Fracturing Pollen Grains ofQuercus RoburL. (Fagaceae)
作者:
Ulf Olsson,
期刊:
Grana
(Taylor Available online 1974)
卷期:
Volume 14,
issue 2-3
页码: 100-102
ISSN:0017-3134
年代: 1974
DOI:10.1080/00173137409429899
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
A new method of sectioning pollen grains by means of laser radiation is described. The grains applied to a specimen stub are exposed to ruby laser rays and subsequently examined under a scanning electron microscope (SEM). The pollen grains are split into pieces of different size suitable for an examination of structural elements. The practical value of the method is discussed. Data of the ruby laser apparatus: ruby crystal 50×5 (Ø) mm, xenon flash tubes, pulses of red light (6943 Å), pulse energy 2.0 J, pulse length 350 μs, peak power 40 kW, average power 5.7 kW, semipermeable mirror (reflection 36%), lens (F= 190 mm).
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