High Performance Liquid Chromatographic Assay of Methoclopramide in Plasma Using a Silica Gel Column and An Aqueous Meobile Phase
作者:
RobertJ.Y. Shi,
WinniaL. Gee,
RogerL. Williams,
EmitT. Lin,
期刊:
Analytical Letters
(Taylor Available online 1987)
卷期:
Volume 20,
issue 1
页码: 131-140
ISSN:0003-2719
年代: 1987
DOI:10.1080/00032718708082241
出版商: Taylor & Francis Group
关键词: HPLC;Matoclopramide;Silice Gal Column;Aqueous Mobile Phase
数据来源: Taylor
摘要:
A high performance liquid chromatographic method for quantitating matoclopramide in plasma is presented. Proteins ara precipitated from the plasma sample with acetonitri la containing the internal standard, procainamida. The treated samples ara than analyzad using an Ultrasphere Si column, an aqueous solution at pH 7 of 65% CH3CN and 5.0 mM (NH4)2HPO4as a mobile phase, and a fluorescence detector. The retention times for drug and intarna1 standard ara 11.2 and 13.2 min, respectively. The caibration curve is Linear from 0.89 to 44.5 ng/ml. The detection limit is 0.89 ng/ml [signaL/hoisa = 31] for 0.2 ml plasma samples Pracision is measured by intraday and intarday coefficients o f variation, which are less than 10%. This method is currently being used for pharmacokinetic studies of methoclopramide.
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