Feeding of defatted millet (Sorghum vulgarie) at different protein levels to rats for 6 weeks caused significant increases in liver total lipids, cholesterol, fatty acids, triglycerides (TG), phospholipids and its two major fractions PC and PE, as compared to the controls. Plasma triglycerides in millet protein fed rats were not reduced. Incorporation of palmitate-1-14C into liver TG, PC and PE of millet protein fed rats was significantly greater than the controls. The incorporation of palmitate-1-14C into plasma TG and into liver FC and EC of rats fed millet protein was equal to or slightly increased as compared to controls. These results show that the accumulation of TG in liver of rats fed millet at different protein levels results from their greatly enhanced synthesis and unimpaired secretion into plasma.