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Separation of micro‐organisms from meat and their rapid enumeration using a membrane filtration‐epifluorescent microscopy technique

 

作者: Isabel Walls,   J. J. Sheridean,   R. W. Welch,   D. A. McDowell,  

 

期刊: Letters in Applied Microbiology  （WILEY Available online 1990）
卷期: Volume 10, issue 1  

页码: 23-26

 

ISSN:0266-8254

 

年代: 1990

 

DOI:10.1111/j.1472-765X.1990.tb00086.x

 

出版商: Blackwell Publishing Ltd

 

数据来源: WILEY

 

摘要:

A new method of separating bacteria from beef mince has been developed, in which an alkaline protease, Alcalase 0.6 L, was used to degrade the meat proteins, leaving micro‐organisms in suspension. The organisms were then counted, using a membrane filtration‐epifluorescent microscopy technique. A correlation coefficient of 0.9? was obtained between this method and the standard plate count, indicating its suitability for use in quality cont

 

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