Diurnal rhythms of hepatic mRNA activities for integral membrane proteins, as determined byin vitrotranslation in a cell‐free system from rat liver
作者:
H.F. Krug,
R. Hardeland,
期刊:
Journal of Interdisciplinary Cycle Research
(Taylor Available online 1981)
卷期:
Volume 12,
issue 1
页码: 35-40
ISSN:0022-1945
年代: 1981
DOI:10.1080/09291018109359723
出版商: Taylor & Francis Group
关键词: Circadian rhythms;liver;membranes;protein synthesis;rat
数据来源: Taylor
摘要:
We investigated the diurnal rhythmicity ofin vitrotranslated mRNAs for integral membrane proteins. Microsomes were isolated from rat liver in different phases of the 24‐h cycle. The polysomal mRNA was translated in a hepaticin vitrosystem containing tRNA, pH 5 enzyme, Sephadex G‐25 eluate, an ATP regenerating system, GTP, spermidine, controlled concentrations of amino acids, and3H leucine. After translation, the membranes were freed from extrinsic proteins by treatment with EDTA and high LiCl. Integral proteins were extracted from the membrane vesicles by SDS, and separated by polyacrylamide gel electrophoresis.3H leucine incorporation into various individual integral membrane proteins revealed diurnal rhythms in the corresponding mRNA activities.
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