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Simple Determination of Steroid Sulfatase Activity in Enzyme Preparation

 

作者: Y. Yamaguchi,  

 

期刊: Analytical Letters  （Taylor Available online 1981）
卷期: Volume 14, issue 17-18  

页码: 1515-1523

 

ISSN:0003-2719

 

年代: 1981

 

DOI:10.1080/00032718108081477

 

出版商: Taylor & Francis Group

 

关键词: steroid sulfatase;serum;chromogenic assay hydrolysis of sulfate ester

 

数据来源: Taylor

 

摘要:

Simple one tube method for the determination of steroid sulfatase activity in an enzyme preparation or commercially available preparation is described. Principle of the reaction is as follows; dehydroepi-androsterone sulfate (DHEA-S) as substrate is hydrolyzed by the catalysis of sulfatase and DHEA released is determined by the enzymic method using 3β-hydroxysteroid oxidase.

 

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