Localization and Production of Plasminogen Activator Inhibitor‐1 in Human Healthy and Atherosclerotic Arteries
作者:
Florea Lupu,
Gabriela Bergonzelli,
Dominik Heim,
Elisabeth Cousin,
Claude Genton,
Fedor Bachmann,
Egbert Kruithof,
期刊:
Arteriosclerosis and Thrombosis: A Journal of Vascular Biology
(OVID Available online 1993)
卷期:
Volume 13,
issue 7
页码: 1090-1100
ISSN:1049-8834
年代: 1993
出版商: OVID
数据来源: OVID
摘要:
High plasma levels of plasminogen activator inhibitor type1 (PAI-1), the principal inhibitor of the fibrinolytic system, have been associated with thrombotic and arterial disease. To study PAI-1 expression in healthy and atherosclerotic human arteries, a detailed analysis was made by light and electron microscopy immunocytochemishy and by in situ hybridization. In healthy arteries PAI-1 was found both at the level of endothelial cells and of smooth muscle cells (SMCs) of the arterial media. In early atherosclerotic lesions PAI-1 was also detected in intimal SMCs and in extracellular areas in association with vitronectin. Immunogold analysis by electron microscopy revealed PAI-1 in vesicular structures in endothelial cells and in SMCs with normal or foam cell characteristics. In advanced atheromatous plaques, PAI-1 mRNA expression in SMCs within the fibrous cap was increased compared with SMCs located in the adjacent media or in normal arterial tissue. PAI-1 mRNA was also detected in macrophages located at the periphety of the necrotic core. The increased synthesis of PAI-1 by cellular components of the atherosclerotic plaque and the extracellular accumulation of PAI-1 may contribnte to the thrombotic complications associated with plaque rupture and possibly play a role in the accumulation of extracellular matrix deposits.
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