Phenylthiohydantoin Formation as a Novel Mode of Assay, Applied to S-Carboxymethylcysteine in Plasma
作者:
E. Reid,
T. McDonald,
J.S. Burton,
期刊:
Analytical Letters
(Taylor Available online 1981)
卷期:
Volume 14,
issue 8
页码: 615-627
ISSN:0003-2719
年代: 1981
DOI:10.1080/00032718108055474
出版商: Taylor & Francis Group
关键词: S-carboxymethylcysteine;carbocysteine;assay;high pressure liquid chromatography (HPLC);phenylthiohydantoin derivative;plasma
数据来源: Taylor
摘要:
The novel method now described, forS-carboxymethylcysteine in plasma at levels that may be lower than 20 μg/ml in therapeutic investigations, entails conversion into the corresponding phenylthiohydantoin (PTH) derivative, so as to confer ultraviolet detectability in a final separation step entailing reverse-phase HPLC. The conditions adopted take account of possible losses of the compound, associated with capricious water-solubility, of stability problems unexpectedly encountered with its PTH derivative, and of the risk of interferences from plasma constituents.
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