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Simultaneous Identification and Determination of Several Barbiturates in Plasma by Reverse-Phase HPLC

 

作者: M. López-rivadulla,   P. Fernández,   P. Rodríguez,   A.M. Bermejo,   L. Concheiro,  

 

期刊: Analytical Letters  （Taylor Available online 1988）
卷期: Volume 21, issue 12  

页码: 2253-2262

 

ISSN:0003-2719

 

年代: 1988

 

DOI:10.1080/00032718808059907

 

出版商: Taylor & Francis Group

 

关键词: HPLC;Barbiturates

 

数据来源: Taylor

 

摘要:

A Reverse-Phase HPLC method is described for the simultaneous identification and determination of 5 barbiturates: Allobarbital, Phencbarbital, Butabarbi -tal, Butalbital and Pentobarbital. Barbital is used as internal standard. The mobile phase is Milli Q Water/ Acetonitrile/ 1.75 M Phosphoric Acid (738:200:2, v/v/v) at a flow rate of 0.8 mL/min and UV detection is carried out at 195 nm. The single extraction procedure requires only small samples and analysis is quick.

 

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