Peptide vaccine candidates against classical swine fever virus: T cell and neutralizing antibody responses of dendrimers displaying E2 and NS2–3 epitopes
作者:
Marta Monsó,
Joan Tarradas,
Beatriz G. de la Torre,
Francisco Sobrino,
Llilianne Ganges,
David Andreu,
期刊:
Journal of Peptide Science
(WILEY Available online 2011)
卷期:
Volume 17,
issue 1
页码: 24-31
ISSN:1075-2617
年代: 2011
DOI:10.1002/psc.1292
出版商: John Wiley&Sons, Ltd.
关键词: classical swine fever;dendrimeric peptides;peptide vaccines;PEG‐like flexibilizing units
数据来源: WILEY
摘要:
AbstractThree peptide‐based systems integrating B and T antigenic sites of CSFV and displaying the B epitopes in fourfold presentation have been designed and produced, and shown to bring about significant enhancements in immunogenicity over the peptides in monomeric form. Of the different strategies tested for producing the dendrimeric constructs, stepwise SPPS using 3,6‐dioxaoctanoic acid as flexible, PEG‐like spacer units at the branching points is clearly advantageous, in particular over ligation in solution. The constructs have been used for immunization of domestic pigs, in order to evaluate the protective response induced by each peptide constructs, and to characterize the B‐ and T‐cell response against CSFV in the natural host. Copyright © 2010 European Peptide Society and John Wiley
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