Parametric sensitivity of stoichiometric flux balance models applied to wild‐typeEscherichia colimetabolism
作者:
Amit Varma,
Bernhard O. Palsson,
期刊:
Biotechnology and Bioengineering
(WILEY Available online 1995)
卷期:
Volume 45,
issue 1
页码: 69-79
ISSN:0006-3592
年代: 1995
DOI:10.1002/bit.260450110
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: E. coli;linear optimization;metabolic fluxes;stoichiometry;sensitivity analysis
数据来源: WILEY
摘要:
AbstractStoichiometrically based flux balance models provide a method to quantify the metabolic pathway fluxes within a living cell. Predictions of flux balance models are expected to have applications in pathway engineering as well as in bioprocess design and control. These models utilize optimality principles applied to metabolic pathway stoichiometry along with the metabolic requirements for growth to determine the flux distribution in a metabolic network. A flux balance model has been developed forEscherichia coliW3110 using five experimentally determined strain‐specific parameters. In this report, we determine the sensitivity of the predictions of the flux balance model to these five strain‐specific parameters. Model predictions are shown to be sensitive to the two parameters describing metabolic capacity, while they are relatively insensitive to the three parameters that describe the metabolic requirements for growth. Thus, when stoichiometrically based models are formulated for additional strains one needs to measure the metabolic capacity (maximum rates of nutrient and oxygen utilization) accurately. Determination of metabolic capacity from batch experiments is relatively easy to perform. On the other hand, the harder to determine maintenance parameters need not be as accurately determined. © 1995 John Wiley&Sons,
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