Kinetic Modelling of in Vitro Lipid Peroxidation Experiments - 'Low Level' Validation of a Model of in Vivo Lipid Peroxidation
作者:
SalvadorArmindo,
AntunesFernando,
PintoRuy E.,
期刊:
Free Radical Research
(Taylor Available online 1995)
卷期:
Volume 23,
issue 2
页码: 151-172
ISSN:1071-5762
年代: 1995
DOI:10.3109/10715769509064029
出版商: Taylor&Francis
关键词: oxidative stress;antioxidants;α-tocopherol;ascorbate;free radicals;computer simulation
数据来源: Taylor
摘要:
Kinetic modelling overcomes some of the drawbacks of purely intuitive thinking in integrating information accumulated on chemical reactions involved in oxidative stress. However, it is important to assess if current knowledge about the reactions that mediate lipid peroxidation already allows satisfactory modelling of this process in near-to-physiological conditions. In this paper, a set of increasingly complexin vitroexperiments on antioxidants (a-tocopherol and ascorbate) and lipid peroxidation in heterogeneous systems is simulated. Quantitative to semiquantitative agreement is found between experimental and simulation results. In addition, this theoretical analysis provided useful insights, suggested new hypotheses and experiments and pointed out relevant aspects needing further research. The results encourage and serve as partial validation for the formulation of relatively detailed mathematical models ofin vivolipid peroxidation. Some important aspects of the formulation and analysis of such models are discussed.
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