Epon Resin Infiltration and Immunogold Labelling of Pituitary Secretory Granules after Cryofixation versus Chemical Fixation
作者:
EneströmSverker,
KniolaBarbara,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1992)
卷期:
Volume 67,
issue 2
页码: 100-105
ISSN:1052-0295
年代: 1992
DOI:10.3109/10520299209110016
出版商: Taylor&Francis
关键词: pituitary granules;cryofixation;chemical fixation;Epon infiltration;immunoelectron microscopy
数据来源: Taylor
摘要:
The degree of infiltration of epoxy resin into pituitary secretory granules was evaluated using X-ray microanalysis of the concentrations of chlorine in the epoxy resins. The effectiveness of infiltration was tested after three different tissue preparation techniques: cryofixation + freeze-drying (CF-FD), glutaraldehyde fixation (GF) + chemical dehydration, and no fixation—no dehydration. Signs of marked incomplete infiltration were found in embedded unfixed tissue while the other two techniques showed 80% infiltration. Uneven penetration was seen after CF-FD and GF. The plastic surface demonstrated a mountain-like appearance over the secretory granules after immunocytochemistry of the glutaraldehyde fixed tissue, whereas the CF-FD tissue showed a less furrowed surface. This probably is due to contact with water, which swells those parts of the granules that are unprotected by the plastic embedding medium. Our findings may explain why it is possible to perform immunocytochemistry on Epon embedded tissue.
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