Intracellular Ca2+Mobilization in Cultured Human Mucosal Microvascular Endothelial Cells from the Nasal Inferior Turbinate
作者:
Hiroki Ikeda,
Nobuo Kubo,
Akihiko Nakamura,
Narinobu Harada,
Toshio Yamashita,
期刊:
ORL
(Karger Available online 1996)
卷期:
Volume 58,
issue 6
页码: 320-325
ISSN:0301-1569
年代: 1996
DOI:10.1159/000276863
出版商: S. Karger AG
关键词: Cultured human mucosal microvascular endothelial cells;ATP;Fura-2;Intracellular calcium concentration;Nasal mucosa
数据来源: Karger
摘要:
Human mucosal microvascular endothelial cells (HMMECs) were isolated and cultured from the mucosa of nasal inferior turbinates. These cells were identified by human factor-VIII antigen and UEA-I. We also investigated alterations in intracellular calcium concentration [Ca2+]i, in HMMECs using the Ca2+-sensitive dye fura-2. Depolarization by high K+ (150 mM) induced no changes in [Ca2+]i in HMMECs, thereby indicating the lack of voltage-dependent Ca2+ channels in these cells. Extracellular ATP increased [Ca2+]i in HMMECs both in the presence and absence of extracellular Ca2+. These results suggest that ATP may induce a release of Ca2+ from intracellular stores in HMMECs. Adenosine induced no changes in [Ca2+]i in HMMECs. It is thus likely that HMMECs obtained from the nasal inferior turbinate may carry P2-purinoceptors but not P1-purinoceptors.
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