Hplc Method for Determination of Lidocaine in Admixture with Dextrose Injection
作者:
F.Mauriece Smith,
NoelO. Nuessle,
期刊:
Analytical Letters
(Taylor Available online 1981)
卷期:
Volume 14,
issue 8
页码: 567-575
ISSN:0003-2719
年代: 1981
DOI:10.1080/00032718108055470
出版商: Taylor & Francis Group
关键词: High performance liquid chromatography;lidocaine;dextrose;injection;admixture
数据来源: Taylor
摘要:
A reverse-phase HPLC system using a microparticulate octade-cylsilane column and an acetonitrile-phosphate buffer mobile phase is employed to determine lidocaine in an admixture of lidocaine injection with 5 per cent dextrose injection. The method is accurate over the range of 5 to 12.5 μg on column, as employed in our laboratories, which corresponds to 2 to 5 mg/ml of lidocaine hydrochloride in the admixture solution. It is precise (2.00 to 2.88% coefficient of variation) and has been used in stability studies of lidocaine. The method is also used to quantitate a degradation product of lidocaine, 2,6-dimethylaniline, over the range of 5 to 25 ng on column (corresponding to 2 to 10 μg/ml in the admixture solution) with a precision of 1.82 to 4.50% coefficient of variation. Samples are prepared by dilution in methanol containing the internal standard, procaine hydrochloride, and each chromatogram requires about 4 minutes.
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