Determination of serum metabolic profiles of bile acids by microcolumn liquid chromatography/laser‐induced fluorescence
作者:
F. Andreolini,
S. C. Beale,
M. Novotny,
期刊:
Journal of High Resolution Chromatography
(WILEY Available online 1988)
卷期:
Volume 11,
issue 1
页码: 20-24
ISSN:0935-6304
年代: 1988
DOI:10.1002/jhrc.1240110107
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: Capillary column liquid chromatography;Laser‐induced fluorescence;Serum bile acids
数据来源: WILEY
摘要:
AbstractA method for the determination of individual free and conjugated bile acids in serum using microcolumn liquid chromatography coupled with a laser‐induced fluorescence detector is described. Bile acids are separated into free/glycine‐conjugate and taurine‐conjugate fractions using a Sep‐Pak SIL cartridge. The taurine‐conjugated bile acid fraction is subjected to enzymatic hydrolysis. Subsequently, free and conjugated bile acids are labeled using 4‐(bromomethyl)‐7‐methoxycoumarin as a fluorogenic reagent, producing stable derivatives that can be excited by the 325 nm line of a He/Cd laser. Prior to their fluorimetric detection, the individual components of a bile acid serum profile are separated by reversed‐phase microcolumn liqu
点击下载:
PDF
(404KB)
返 回