Quantitative determination of histamine metabolites by capillary gas chromatography
作者:
H. Navert,
R. Bérubé,
A. Wollin,
期刊:
Canadian Journal of Physiology and Pharmacology
(NRC Available online 1985)
卷期:
Volume 63,
issue 6
页码: 766-772
ISSN:0008-4212
年代: 1985
DOI:10.1139/y85-127
出版商: NRC Research Press
数据来源: NRC
摘要:
A method using capillary gas chromatography is described for the determination of histamine and eight of its basic and acid metabolites in a single biological sample of serum, urine, or gastric juice. Ion-exchange chromatography and extraction with organic solvents are used for isolation and purification, and gas chromatography for identification and quantitation. The heptafluorobutyryl derivatives of histamine and some basic metabolites are compatible with nitrogen–phosphorus and electron capture detection modes and offer an excellent sensitivity (detection limit 0.1 pmol with electron capture). The acid metabolites are quantitated after esterification. The linearity range, the sensitivity, a partial study of reproducibility and typical chromatograms show that the method is adaptable to a variety of applications.
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