Octa-O-acetylsucrose (1) was regioselectively hydrolyzed by the lipase AK from Pseudomonas sp. in aqueous buffer and two hepta-O-acetylsucroses and two hexa-O-acetylsucroses were obtained by column purification. After analysis by NMR methods, four products were shown to be 3,4,6,1′,3′,4′,6′-hepta-O-acetylsucrose (2), 2,3,4,6,1′,3′,6′-hepta-O-acetylsucrose (3), 3,4,6,1′,3′,6′-hexa-O-acetylsucrose (4) and 2,3,4,6,3′,6′-hexa-O-acetylsucrose (5).