Corneal deturgescence during organ culture
作者:
WalkenbachRonald J.,
CorwinJames G.,
期刊:
Current Eye Research
(Taylor Available online 1987)
卷期:
Volume 6,
issue 2
页码: 381-389
ISSN:0271-3683
年代: 1987
DOI:10.3109/02713688709025191
出版商: Taylor&Francis
数据来源: Taylor
摘要:
A simple method is described to monitor rabbit and human corneal deturgescence while the tissue is suspended in tissue culture media. The composition of the media is such that the cornea slowly thickens at 4°but exhibits the classic“temperature reversal”thinning phenomenon when incubated at 23°-37°. Each cornea is cultured in a closed eye bank corneal viewing chamber during swelling and deswelling phases of an experiment, minimizing direct handling of the tissue. Optimal conditions for observing corneal deturgescence are described. This protocol has several advantages over corneal perfusion techniques and could be further developed for routine use in eye banks as a functional test of donor suitability for keratoplasty.
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