Assessment of fluorochromes for cellular structure and function studies by flow cytometry
作者:
Jean‐Michel Petit,
Michelle Denis‐Gay,
Marie‐Hélène Ratinaud,
期刊:
Biology of the Cell
(WILEY Available online 1993)
卷期:
Volume 78,
issue 1‐2
页码: 1-13
ISSN:0248-4900
年代: 1993
DOI:10.1016/0248-4900(93)90109-R
出版商: Blackwell Publishing Ltd
关键词: fluorophores;intracellular calcium;membrane potenlials;glutathione;cyloenzymology
数据来源: WILEY
摘要:
Summary—Because flow cytometry permits the analysis of individual whole cells, one of the key requirements in selecting a probe is its ability to target the site of interest into cells. In addition, dyes must possess ideal properties (ie extinction coefficient, Stoke's shift) rendering them appropriate for this methodology. Other characteristics, such as fluorescence quenching and energy transfer, inherent to the staining, provide numerous applications in flow cytometry. The available fluorophores used in flow cytometry are classified according to their cellular incorporation and binding. Thus, probes are presented and discussed as follows: 1) dyes of cellular components (DNA, RNA, proteins, lipids); 2) probes of membrane potential; 3) fluorophores that are sensitive to their microenvironment (pH, calcium, etc); and 4) those used for measurement of enzymatic activities into cell
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