Eight asphalts selected for intensive study in the Strategic Highway Research Program have been separated into neutral, basic, and acidic fractions by means of ion exchange chromatography. Each asphalt exhibits a characteristic fractionation pattern into neutral, acidic, and basic components. The separations into chemical types were verified by elemental analysis and infrared methods. Separations were reasonably repeatable, and recoveries were acceptable. Large enough amounts of asphalts could be separated so that properties of interest of the fractions could be studied.