Interactions between mucopolysaccharides and cationic polypeptides in aqueous solution: Hyaluronic acid, heparitin sulfate, and keratan sulfate
作者:
R. A. Gelman,
J. Blackwell,
期刊:
Biopolymers
(WILEY Available online 1974)
卷期:
Volume 13,
issue 1
页码: 139-156
ISSN:0006-3525
年代: 1974
DOI:10.1002/bip.1974.360130109
出版商: Wiley Subscription Services, Inc., A Wiley Company
数据来源: WILEY
摘要:
AbstractCircular dichroism spectroscopy has been used to study the interactions of hyaluronic acid, heparitin sulfate, and keratan sulfate with cationic polypeptides. The results indicate that the presence of these mucopolysaccharides has an effect in the conformation of poly(L‐lysine) and poly(L‐arginine), such that the former adopts the “random” form and the latter takes up the α‐helical conformation, rather than the “charged coil” form expected at neutral pH. The relative strengths of the interactions can be judged from the melting temperatures above which they are disrupted. Both the stoichiometry and the strength of the interactions depend on the position, number, and type of anionic groups attached to the polysaccharide backbone. Such considerations place the six common mucopolysaccharides in order of increasing strength of interaction: hyaluronic acid
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