Microprocessor‐controlled photon‐counting spectrofluorometer
作者:
Enrico Gratton,
Mark Limkeman,
期刊:
Review of Scientific Instruments
(AIP Available online 1983)
卷期:
Volume 54,
issue 3
页码: 294-299
ISSN:0034-6748
年代: 1983
DOI:10.1063/1.1137386
出版商: AIP
数据来源: AIP
摘要:
The construction details and performance characteristics for a fluorescence spectrophotometer are described. The system utilizes proton counting for signal detection and an Apple II microcomputer for data acquisition and analysis. Data‐acquisition techniques are given along with typical excitation, emission, and polarization spectra. Particular reference is made to application of the instrument to fluorescence studies of protein/polymer films.
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