Differences in the Biological Activities of Transforming Growth Factor-βand Platelet-Derived Growth Factorin vivo
作者:
OgawaYasushi,
KsanderGeorge A.,
PrattBruce M.,
SawamuraSteven J.,
ZimanJill M.,
GerhardtCarolyn O.,
AvisPaula D.,
MurrayMark J.,
McPhersonJohn M.,
期刊:
Growth Factors
(Taylor Available online 1991)
卷期:
Volume 5,
issue 1
页码: 57-68
ISSN:0897-7194
年代: 1991
DOI:10.3109/08977199109000271
出版商: Taylor&Francis
关键词: TGF-β;PDGF;connective tissue deposition;wound healing
数据来源: Taylor
摘要:
AbstractTransforming growth factor-β1 (TGF-β1 and recombinant platelet-derived growth factor-BB (rPDGF-BB) promoted an extensive, dose-dependent development of fibrous connective tissue when continuously delivered for 8 days by mini-osmotic pumps implanted subcutaneously in adult guinea pigs. Biochemical analyses demonstrated that TGF-β1 and rPDGF-BB stimulated dose-dependent increases in the dry weight, and protein, DNA, collagen, and glycosaminoglycan (GAG) contents of the fibrous connective tissue capsule that enveloped the pumps. The GAG/DNA mass ratio was markedly elevated by TGF-β1, but the collagen/DNA, protein/DNA, and collagen/protein ratios were not significantly increased. In contrast, rPDGF-BB generally decreased these mass ratios. Histological analyses suggested that this was due to the fact that rPDGF-BB induced a very cellular response with a marked influx of neutrophils and fibroblasts. TGF-β1 induced significantly less cellular response, which consisted primarily fibroblasts and macrophages. These results indicated that rPDGF-BB and TGF-β1 induced connective tissue depositionin vivoin a dose-dependent fashion, although the cellular nature of the responses as well as the structural composition of the extracellular matrices were clearly distinguishable between the two growth factors.
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