DETERMINATION OF ADENINE NUCLEOTIDES INMYTILUS GALLOPROVINCIALIS LMK. BY ION-PAIR HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH A DIODE ARRAY DETECTOR
作者:
M.J. Díaz Enrich,
R. Barcia,
J.I. Ramos Martínez,
I. Ibarguren,
期刊:
Journal of Liquid Chromatography & Related Technologies
(Taylor Available online 1999)
卷期:
Volume 22,
issue 9
页码: 1391-1402
ISSN:1082-6076
年代: 1999
DOI:10.1081/JLC-100101740
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
A quick and sensitive method for the determination of AMP, ADP, and ATP levels in mantle tissue of the sea musselMytilus galloprovincialis Lmk. is described. The method uses ion-pair high-performance liquid chromatography with diode array detection, allowing obtaining of the UV absorption spectrum for each peak. Method precision was good. The detection limit for all three nucleotides was 20 pmol (much lower than the minimum concentrations in this tissue).
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