Partial separation of the pituitary proteins ofLabeo umbratus, Smith (mudfish)
作者:
J. Hattingh,
P. J. Toit,
期刊:
Journal of Fish Biology
(WILEY Available online 1973)
卷期:
Volume 5,
issue 1
页码: 41-47
ISSN:0022-1112
年代: 1973
DOI:10.1111/j.1095-8649.1973.tb04429.x
出版商: Blackwell Publishing Ltd
数据来源: WILEY
摘要:
L. umbratuspituitary glands were partially separated by means of preparative polyacry‐lamide electrophoresis and chromatography on DEAE‐cellulose and Sephadex G‐50. Eight fractions were obtaìned and it was found that fraction 1 contained potent adrenocorticotropic stimulating activity, with a molecular weight in the region of 6000. Fractions 3 and 4 contained potent gonadotropic and a lesser degree of exopthalmic producing activity, with molecular weights in the region of 14 000 to 20 000. Fraction 6 displayed pigeon‐crop stimulating activity, with a molecular weight above 30 000. Fractions 7 and 8 displayed M.S.H. and vasopressinoxytocic activity respectively, with a molecular weight in the region of 5500–6500. Fractions 2 and 5 could not be identified. The fractions seemed to be glycoproteins and the results are discussed in relation to previou
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