A wide range of variations was recorded in the somaclones ofMentha arvensisL. var.piperascensHolmes for menthol (65.2–94.8%), menthone (1.4–20.9%), isomenthone (1.0–5.2%) and menthyl acetate (0.8–8.5%). Correlation analysis of these four constituents revealed a negative significant correlation of menthol with menthone (r= −0.856) and isomenthone (r=−0.837), while menthone had a positive significant correlation with isomenthone (r=0.853). Eight somaclones possessed a higher menthol content than the parent, while five of these had also been ranked promising based on the agronomic characters. On the basis of quantitative and qualitative traits, 27 somaclones were further evaluated in a replicated plant to row trial along with the parent clone. A decline in menthol content along with 2–4 fold increase in menthone and isomenthone was observed in a randomized block design trial with somaclones. Two somaclones (Sc 93 and Sc 179) showed stability in terms of percent composition of the volatile oil. This communication provides a procedure for selecting the plants based on oil composition. The performance of these in vitro raised clones in evaluation trial is discussed in relation to the possibility of improving essential oil quality of mints through somaclonal breeding.