ABSTRACTA survey of major polysaccharides occurring in cell walls ofOphiostoma ulmiandVerticillium alboatrumwas performed by means of gold-complexed lectins and glycosidic enzymes. Some differences in wall carbohydrate composition and distribution were noted in these fungi. Both chitin and cellulose were found to occur in the walls ofO. ulmiwhereas only chitin was present in those ofV. albo-atrum.Polygalacturonic acids were identified in both fungi but a greater amount of galactose and N-acetyl-D-galactosamine residues occurred in the walls ofV. albo-atrum.In contrast,α-mannosyl and/orα-glucosyl groups were detected only inO. ulmicell walls.β-glucosides and sialic acid were absent in the walls of both fungi.