Expression of c-kitandkitLigand in Human Colon Carcinoma Cells
作者:
Minoru Toyota,
Yuji Hinoda,
Akinori Takaoka,
Yusuke Makiguchi,
Tohru Takahashi,
Fumio Itoh,
Kohzoh Imai,
Akira Yachi,
期刊:
Tumor Biology
(Karger Available online 1993)
卷期:
Volume 14,
issue 5
页码: 295-302
ISSN:1010-4283
年代: 1993
DOI:10.1159/000217842
出版商: S. Karger AG
关键词: c-kit;kitligand;Human colon carcinoma cells
数据来源: Karger
摘要:
To determine whether c-kit and kit ligand (KL) mRNAs could be expressed in human epithelial tumors, reverse transcrip-tase-polymerase chain reaction and Northern blot analysis were performed. KL mRNA was shown to be expressed in a variety of epithelial tissues and cell lines. The expression of c-kit mRNA was then examined in hepatocellular and colon carcinoma cell lines. While hepatocellular carcinoma cell lines did not express c-kit mRNA as far as we could ascertain, 2 of 5 colon carcinoma cell lines showed the expression of both c-kit and KL mRNAs. Furthermore, the expression of c-kit in these cells was demonstrated at the protein level by flow cytometry. These data suggest that c-kit and KL may play an important role as an autocrine loop in the proliferation of some colon carcinoma cells.
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