Increased enzyme activity in immobilised cell composites with a highly hydrophilic polymer matrix
作者:
Minoru Kumakura,
Isao Kaetsu,
期刊:
Journal of Chemical Technology and Biotechnology. Biotechnology
(WILEY Available online 1984)
卷期:
Volume 34,
issue 1
页码: 39-44
ISSN:0264-3421
年代: 1984
DOI:10.1002/jctb.280340107
出版商: John Wiley&Sons, Ltd.
关键词: Cell immobilisation;radiation copolymerisation;glucose isomerase;Streptomyces phaeochromogenes;polymer hydrophilicity
数据来源: WILEY
摘要:
AbstractStreptomyces phaeochromogenescells were immobilised by radiation polymerisation of hydrophilic monomers; highest levels of glucose isomerase activity were found in immobilised cell composites with highly hydrophilic polymer matrices. The enzyme activity in the composites from the copolymerisation of hydroxyethyl acrylate (or methacrylate) andN‐vinyl‐2‐pyrrolidone monomer increased markedly with increase in the content of theN‐vinyl‐2‐pyrrolidone monomer, owing to the higher water absorption by the polymer matrix, and was close to that of the non‐immobilised cells. The enzyme activity was affected by monomer concentration, the hydrophilicity, and chemical structure of the polymer matrix. The Michaelis constant of the composites from hydrophilic monomers was close to that of the
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