Calmodulin Inhibitors Decrease the CRF- and AVP-Induced ACTH Release in vitro: Interaction of Calcium-Calmodulin and the Cyclic AMP System
作者:
Kazuharu Murakami,
Kozo Hashimoto,
Zensuke Ota,
期刊:
Neuroendocrinology
(Karger Available online 1985)
卷期:
Volume 41,
issue 1
页码: 7-12
ISSN:0028-3835
年代: 1985
DOI:10.1159/000124146
出版商: S. Karger AG
关键词: Calmodulin inhibitors;Adrenocorticotropic hormone release;Synthetic ovine corticotropin-releasing factor;Arginine vasopressin;Cyclic AMP
数据来源: Karger
摘要:
The effect of N-(6-aminohexyl)-5-chloro-naphthalene-l-sulfomide (W-7) and trifluoperazine (TFP) was examined on ACTH release from cultured rat anterior pituitary cells and pituitary halves. These drugs significantly inhibited the ACTH release induced by synthetic ovine corticotropin-releasing factor (CRF) in a dose-related manner. In pituitary halves, arginine vasopressin (AVP) at 10 and 100 ng/ml showed almost the same ACTH-releasing activity as CRF at the same concentrations. W-7 and TFP inhibited the CRF- and AVP-induced ACTH release from pituitary halves. The cyclic AMP levels in the pituitary halves were significantly increased by CRF, but not AVP. Although W-7 inhibited CRF-in-duced ACTH release, it did not have an effect in cyclic AMP accumulation. These results suggest that CRF exerts ACTH-releasing activity through both the calcium-calmodulin system and cyclic AMP system and that AVP stimulates ACTH release mainly through the calcium-calmodulin system.
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