Performance in endurance activities depends on maximal aerobic capacityand the ability to sustain a high percentage ofover time. This study examined whether noninvasive laboratory measures would be valid predictors of endurance performance in an individual-start bicycle race (TT). Eight experienced male cyclists (age = 25.1 ± 3.3 years, weight = 75.0 ± 5.7 kg,) performed a progressive incremental exercise test to exhaustion on a cycle ergometer., maximum power output, and ventilatory threshold were determined. Later the subjects completed a 40-km TT. Power output at the ventilatory threshold (VT watts) was correlated with race performance time and calculated power output during the competition (r = −0.81; r = 0.82). VT watts andaccounted for 75% of the variance between subjects (r = 0.91) in performance time. These data indicate that simple laboratory measures can predict TT performance in trained cyclists. Individual differences may be accounted for by motivation, aerodynamic position, and efficiency.Key words:exercise, laboratory methods,, athletes