A Versatile Method for Analysis of Bile Acids in Plasma
作者:
Ikuko Makino,
J. Sjövall,
期刊:
Analytical Letters
(Taylor Available online 1972)
卷期:
Volume 5,
issue 6
页码: 341-349
ISSN:0003-2719
年代: 1972
DOI:10.1080/00032717208066109
出版商: Taylor & Francis Group
关键词: bile acids;plasma;liquid-solid extraction;gas chromatography
数据来源: Taylor
摘要:
Plasma or serum is diluted ten times with 0.1 M NaOH. Bile acids are extracted and purfied by passing the sample through a column of Amberlite XAD-2. The resin adsorbs the bile acids, which are then eluted with ethanol. After addition of sodium hydroxide and hydrolysis, the free bile acids are extracted by Amberlite XAD-2 and are then methylated and analyzed by gas chromatography. The degree of purification obtained in the first extraction step is sufficient to permit an analysis of conjugated bile acids or bile acid sulfates by thin-layer or Sephadex LH-20 chromatography.
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