Sex‐peptide activates juvenile hormone biosynthesis in theDrosophila melanogastercorpus allatum
作者:
Pnina Moshitzky,
Irène Fleischmann,
Nivi Chaimov,
Philippe Saudan,
Stefan Klauser,
Eric Kubli,
Shalom W. Applebaum,
期刊:
Archives of Insect Biochemistry and Physiology
(WILEY Available online 1996)
卷期:
Volume 32,
issue 3‐4
页码: 363-374
ISSN:0739-4462
年代: 1996
DOI:10.1002/(SICI)1520-6327(1996)32:3/4<363::AID-ARCH9>3.0.CO;2-T
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: juvenile hormone;sex peptide;corpus allatum;female receptivity;ovulation;oviposition;Drosophila melanogaster
数据来源: WILEY
摘要:
AbstractMating elicits two well‐defined reactions in sexually matured females of many insects: reduction of receptivity and increased oviposition. These post‐mating responses have been shown to be induced by factors synthesized in the reproductive tract of the adult male and transferred in the seminal fluid to the female during copulation. One of these factors, named sex‐peptide (SP), has been identified inDrosophila melanogaster.Using an in vitro radiochemical assay, we show that synthetic sex‐peptide considerably activates juvenile hormone III‐bisepoxide (JHB3) synthesis in corpus allatum (CA) excised from Days 3 and 4 post‐eclosion virgin females. Base levels are significantly lower at emergence (Day 0) than on subsequent days, and only weak stimulation is obtained on Day 1, while none is obtained on Day 2, where maximal basal synthesis occurs. The CA of mated females cannot be stimulated further for at least 7 days, but regain responsiveness by Day 10 after mating. Synthesis of JHB3stimulated by SP in vitro persists for at least 4 h after removal of the peptide. Development of responsiveness of the CA to SP in vitro is compared with development of the post‐mating reactions of sex‐peptide injected virgin females. Our results suggest that the CA is a direct target for SP in vivo and that sexual maturity is established separately for the two post‐mating reactions. © 19
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