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Identification of Somatomammotroph Cells in Lactating Goats(Capra hircus)by Fluorescence and Immunogold Techniques

 

作者: J. Sánchez,   J.A. Navarro,   A. Bernabé,   S. Gómez,   B. Garcés,   M.A. Gómez,  

 

期刊: Cells Tissues Organs  (Karger Available online 1994)
卷期: Volume 149, issue 1  

页码: 39-45

 

ISSN:1422-6405

 

年代: 1994

 

DOI:10.1159/000147553

 

出版商: S. Karger AG

 

关键词: Adenohypophysis;Somatomammotroph cells;Immunogold;Fluorescence;Goat

 

数据来源: Karger

 

摘要:

The occurrence and ultrastructural morphology of somatomammotroph cells, in the anterior pituitary tissue of the goat, is studied using fluorescence microscopic double staining and two methods of double immunogold staining combined with electron microscopy. Under the fluorescence microscope numerous monohormonal cells, either mammotroph cells (prolactin, PRL, cells) or somatotroph cells (growth hormone, GH, cells) are observed, but cells containing both hormones (somatomammotroph or SMT cells) are scarce. SMT cells do not differ ultra-structurally from monohormonal cells, and the cytoplasm contains two populations of granules, each reactive only for one of the antisera, either antisheep GH or antisheep PRL.

 

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