An adaptability of countercurrent chromatographic data to Van Deemter equation (H = A + B/u + Cu) is examined under a fixed volume of the stationary phase. Three test samples, epigallochatechin gallate (EGCG), gallocatechin-gallate (GCG), and epicatechin gallate (ECG) were separated using a two-phase solvent system of n-hexane/ethyl acetate/water (3.5:1:10, v/v/v) at various linear flow rates (u).