Monoclonal Antibody M3 Used in Tissue Polypeptide–Specific Antigen Assay for the Quantification of Tissue Polypeptide Antigen Recognizes Keratin 18
作者:
Johannes M.G. Bonfrer,
Els M. Groeneveld,
Catharina M. Korse,
Arie van Dalen,
Lauran C.J.M. Oomen,
Dagmar Ivanyi,
期刊:
Tumor Biology
(Karger Available online 1994)
卷期:
Volume 15,
issue 4
页码: 210-222
ISSN:1010-4283
年代: 1994
DOI:10.1159/000217894
出版商: S. Karger AG
关键词: Monoclonal antibodies;Assay;Cytokeratins;Cell lines
数据来源: Karger
摘要:
Recently, a new ‘specific tissue polypeptide antigen (TPA)’ test was introduced and designated tissue polypeptide-specific antigen (TPS); it is based on the monoclonal antibody (MAb) anti-TPS, M3. We have tested the specificity of this antibody by immunocyto- and immunohistochemistry, gel electrophoresis and immunoblotting. MAb M3 bound to intermediate filaments of epithelial cells and revealed a staining pattern identical to cytokeratin (CK) 18-specific MAb (DE-K18) on tissue sections of various human tissues. On immunoblots of proteins extracted from various epithelial cell lines, M3 reacted with a 45-kD protein corresponding to CK18, and on immunoblots of proteins isolated from MCF-7 culture fluid M3 stained three bands, 45, 33 and 29 kD. The same bands were stained with CK18-specific MAb, indicating that they represent CK18 and its degradation products. TPA, used as a tumor marker in clinical diagnoses and follow-up, was shown to be a degradation product of CK 8, 18 and 19. In contrast to TPA, MAb M3 did not stain CK8 and CK19 present on immunobl
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