FluorescentPseudomonasspp. from 62 root, leaf, and fruit samples covering 24 horticultural crops, and 8 pastoral soil samples, were tested in vitro for their ability to inhibit the growth of five pathogenic fungi—Botrytis cinereaPersoon,Fusarium oxysporumSchlechtendal f. sp.lycopersici(Saccardo) Snyder & Hansen,Phytophthora nicotianaevan Breda de Haan varnicotianae, Pythium debaryanumauct. (non Hesse), andPythium ultimumTrow. Bacterial isolates from 17, 15, 10, and 5 crops showed some degree of antagonism towardsPythiumspp.,B. cinerea, P. nicotianae,andF. oxysporumrespectively. The isolates from the rhizosphere of beetroot, lettuce, loquat, pasture, strawberry, and the fructoplane of lemon had the greatest percentage of antifungal bacteria. There were differences between plant species in the percentage of antifungalPseudomonasspp. isolates showing antifungal activity againstPythium ultimum (P <0.001),P. debaryanum(P<0.01),P. nicotianae(P<0.01), andB. cinerea (P<0.05).Antifungal activity against at least two pathogens was shown by 9% of the isolates.