In experiments with dogs (n = 610), the influence of temperature, flow rate, perfusate, and perfusion duration on the hind leg subjected to an isolation perfusion ads studied. The perfusion pressure, the blood gases, and the metabolic status in specimens of skeletal muscle obtained at the end of the perfusion period served us parameters. A perfusion of 1 and 2 h with whole blood, a flow rate of 10 mL/100 g/min, rind temperatures of up to 42°C did not result in alterations of the energy metabolism. When the flow rate was lowered to 5 mL/100 g/min, when other perfusates were used, or when the im temperature was raised to 43.5°C, then tissue damage occurred Knowledge gained in these. Extremities was utilized in the treatment of 371 patients with malignancies of the extremities, and an extremely low complication rate was observed.