A sensitive adsorptive stripping voltammetric method for measurement of hesperidin in 0.1 M NaAc-HAc solution was described. The method was based on the adsorptive accumulation of the drug at a hanging mercury drop electrode, followed by linear sweep voltammetry. The response was evaluated with respect to preconcentration time, pH effect and concentration of HAc-NaAc buffer solution. The peak potential was -1.01V (vs.Ag/AgCl). The peak current was directly proportional to the concentration of hesperidin with a detection limit of 3.0×10−7M at an accumulation time of 2 min. The reduction process is irreversible and the wave shows adsorptive characteristics.