A Simple Staining Procedure for Detecting the true Acrosome Reaction in Buffalo(Bubalus bubalis)Spermatozoa
作者:
SidhuK. S.,
DhindsaJ. S.,
GurayaS. S.,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1992)
卷期:
Volume 67,
issue 1
页码: 35-39
ISSN:1052-0295
年代: 1992
DOI:10.3109/10520299209110003
出版商: Taylor&Francis
关键词: capacitation;acrosome reaction;acrosome;postnuclear cap
数据来源: Taylor
摘要:
A simple dual staining procedure for detecting the true acrosome reaction in dried smears of buffalo spermatozoa is described. Trypan blue is used first to differentiate live from dead spermatozoa and the dried smears which have been prepared are stained with Giemsa for acrosome evaluation. Four categories of spermatozoa were recognized: A) live, intact acrosome (acrosome pink, postnuclear cap clear); B) dead, intact acrosome (acrosome pink, postnuclear cap blue); C) live, detached acrosome (acrosome clear, postnuclear cap clear); and D) dead, detached acrosome (acrosome clear, postnuclear cap blue). The procedure is simple, rapid and convenient for assessing true acrosome reaction in buffalo spermatozoa. Simultaneous assessment of sperm viability and its acrosomal status in dried smears makes this procedure attractive because the true acrosome reaction can be studied thoroughly at a later state after the incubation period.
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